By Peggy Geluykens, Koen Van Acker, Johan Vingerhoets, Christel Van den Eynde (auth.), Edwin Yunhao Gong (eds.)

Although antiviral medications were effectively built for a few viral ailments, there is still a transparent, unmet clinical have to enhance novel antiviral brokers for the keep an eye on and administration of many viruses that at the moment don't have any or constrained cures in addition to a necessity to beat the restrictions linked to the present antiviral medicines, resembling hostile results and emergence of drug-resistant mutations. the second one version of Antiviral equipment and Protocols features:

  • All chapters are new and written by way of specialists within the box, reflecting the most important contemporary technical advances in antiviral examine and discovery.
  • This variation makes a speciality of many vital human viruses, resembling human immunodeficiency virus style 1 (HIV-1), hepatitis viruses (hepatitis B and C viruses), herpes viruses, human breathing syncytial virus (RSV), and influenza virus, whereas additionally that includes a few vital rising viruses, comparable to dengue virus, West Nile virus, and chikungunya virus.
  • As a quantity within the hugely winning Methods in Molecular Biology sequence, chapters comprise introductions to their respective issues, lists of the mandatory fabrics and reagents, step by step, effortlessly reproducible laboratory protocols, and pointers on troubleshooting and averting recognized pitfalls.

Comprehensive and state-of-the-art, Antiviral equipment and Protocols, moment Edition will function a very good laboratory reference for pharmaceutical and educational biologists, medicinal chemists, and pharmacologists in addition to for virologists within the box of antiviral learn and drug discovery.

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Key words HIV-1, Genotyping, Resistance testing, Resistance-associated mutations, Reverse transcriptase inhibitors, Protease inhibitors, Integrase inhibitors, vircoTYPE™ HIV-1 1 Introduction During the last decade, significant progress has been made in managing human immunodeficiency virus (HIV-1)-infected patients [1, 2]. ), Antiviral Methods and Protocols, Methods in Molecular Biology, vol. 1007/978-1-62703-484-5_5, © Springer Science+Business Media, LLC 2013 37 38 Peter Van den Eede et al. by the rapid evolution of viral resistance, which was considered an inevitable consequence of antiretroviral (ARV) therapy.

Clear Seal 3730 (Applied Biosystems). 11. 96-well PCR plate (ABgene). 12. ABI 3730XL Analyzer (Applied Biosystems). 13. ). 14. BioEdit v7 (Ibis Biosciences). 2 Extraction of Viral RNA 1. Take the buffers out from 4 °C and ensure that they are at room temperature prior to use. 2. Invert the bottles containing the solution several times to ensure that all crystals are dissolved. 3. Thaw the samples and spin down before opening the tubes. 4. Switch on the NucliSens® easyMAG® instrument and computer (see Note 1).

Acta Pharmacol Sin 28:811–817 Grobler JA, Stillmock KA, Hazuda D (2009) Scintillation proximity assays for mechanistic and pharmacological analyses of HIV-1 integration. Methods 47:249–253 John S, Fletcher TM III, Jonsson CB (2005) Development and application of a highthroughput screening assay for HIV-1 integrase enzyme activities. J Biomol Screen 10:606–614 Farnet CM, Wang B, Lipford JR et al (1996) Differential inhibition of HIV-1 preintegration complexes and purified integrase protein by small molecules.

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