By J. B. Clarke, U. Kreuzberg-Duffy, C. MacDonald, H. Moulsdale, J. Golding, B. Griffiths (auth.), E. C. Beuvery, J. B. Griffiths, W. P. Zeijlemaker (eds.)
Animal phone expertise is a self-discipline of turning out to be value, which goals now not only at knowing constitution, functionality and behavior of differentiated animal cells, yet in particular on the improvement in their skills worthwhile for scientific program. subject matters of curiosity during this regard contain: viral vaccines, pharmaceutical proteins and novel purposes corresponding to gene treatment and organ tradition.
certainly, those lawsuits of the joint assembly of the ecu Society for Animal mobile know-how and the japanese organization for Animal mobile expertise (Veldhoven, The Netherlands, September 1994) evaluation the latest prestige of the sector, and may be most respected to someone actively interested in the tradition of animal cells and its purposes. The contributions to this quantity have been strictly chosen at the foundation of caliber and novelty of contents.
Kluwer is honoured which will upload this paintings to its strongly constructing booklet programme in mobile and tissue tradition, which now has its connections to all significant Societies during this box all over the world.
Audience: phone biologists, biochemists, molecular biologists, immunologists, virologists and all different disciplines relating to animal cellphone know-how, operating in a tutorial surroundings, in addition to in (biotechnology or pharmaceutical) industry.
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Extra info for Animal Cell Technology: Developments Towards the 21st Century
Despite their disadvantages in more complex genetic manipulation and more demanding growth physiology, cultured mammalian cells have the advantage that their products are authentic - that is, they process target proteins through the normal secretory pathway, so folding, assembly, and posHranslational modification occur before secretion. In the past, a great deal of work has been done to optimise the production of proteins by vector and process engineering. The aim of this study is to understand and, if possible, manipulate the intracellular processes involved in the efficient secretion of authentic mammalian proteins.
Dil Haemaggluthaemadsorption inating titre visible at 1110 dil. 0 (0) 13 27200(23241 ) 13 Acknowlegements The authors are grateful to the UK Public Health Laboratory service for financial support, and to Dr J. Stevenson and Mr A. Fooks for advice and use of facilities. References 1. Clarke, J. B. (1994). Immortalised cell lines for virus diagnosis. In: Animal cell technology: Developments, processes, and products. (Eds. , Oxford, pp 661-663. 2. B. (1994) Immortalised cell lines for virus diagnosis.
Virus was then titrated on 293 cells. 3. Results and Discussion Electron microscopy of MRC5 cells shows that nucleocapsid-like structures are clearly visible in the cytoplasm and nucleus of cells infected with RAd68 (Fig 1), which correlates with previous data from insect cells (9). Expression of the MV N protein in MRC5 cells can be detected by immunofluorescence (IF) analysis of cells infected with RAd68 (data not shown). IF analysis shows that both the cytoplasm and nucleus of the infected cells contains antigenic N protein, whi~ is in agreement with the EM data.